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Sirius Transfection Reagent
产品概述：
Sirius Transfection Reagent
(P40)
I-Application：
Sirius Transfection Reagent is a proprietary formulation for transfectin nucleic aicids into a wide range of eukaryotic cells . It is designed to transfect a wide range of eukaryotic cells including insect cells and many cell lines not transfected well by other reagents （e.g .,MCF-7, RAW 264.7, PC-3, HeLa, MA-10, HepG2, SH-SY5Y, A7r5, STO, SCC-61, STSAR-90, SQ20B, T98）.
Note: For the most up-to-date list of cell types that have been successfully transfected with Sirius Transfection Reagent for life science research only.
II-Product Description：
Sirius Transfection Reagent is a multi-component reagent that forms a complex with DNA and siRNA , then transports the complex insect cells.
Storage: Store at 4°C (Don’t Freezing).
III-Functional analysis:
Cos7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.2-0.5 µl Sirius Transfection Reagent and 0.1 µg DNA or 0.25 µlSirius Transfection Reagent and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be 70% - 90% compared to a reference lot in at least one of the tested amounts, listed above.
Procedure Details | ||||
Component | 96-well | 24-well | 12-well | 6-well |
Adherent cells | 1–4 × 104 | 0.5–2 × 105 | 1–4 × 105 | 0.25–1 × 106 |
Opti-MEM® Medium | 25 μL × 4 | 50 μL × 4 | 100 μL × 4 | 150 μL × 4 |
Sirius Transfection Reagent | 1, 1.5, 2, 2.5 μL | 2, 3, 4, 5 μL | 4,6,8, 10ul | 6, 9, 12, 15 μL |
Opti-MEM® Medium | 125 μL | 250 μL | 500ul | 700 μL |
DNA (0.5–5 μg/μL) | 2.5 μg | 5μg | 10ug | 14 μg |
Diluted DNA Total | 25 μL | 50 μL | 100ul | 150 μL |
Diluted Sirius Transfection Reagent | 25 μL | 50 μL | 100ul | 150 μL |
Incubate for 5 minutes at room temperature. | ||||
Component | 96-well | 24-well | 12-well | 6-well |
DNA-lipid complex per well | 10 μL | 50 μL | 100ul | 250 μL |
Final DNA used per well | 100 ng | 500 ng | 1000ng | 2500 ng |
Final Sirius Transfection Reagent used per well | 0.2–0.5 μL | 1.0–2.5 μL | 2.0-5.0ul | 5.0–12.5 μL |
Incubate cells for 1–3 days at 37°C. Then analyze transfected cells. | ||||
IV- Cytotoxicity analysis:
Cell growth is assessed using the Cell Counting Kit (K009,ZETA LIFE Inc.)which is an indirect measure of cytotoxicity. The remainder of cells from functional analysis is tested for viability.
V-The important guidelines：
Sirius Transfection Reagent complexes must be made in serum-free medium such as Opti-MEM Reduced serum Medium and can be added directly to cells in culture medium, in the presence or absence of serum .
It is not necessary to remove complexes or change and add medium after
transfection.The amount of Sirius Transfection Reagent required for successful transfection varies depending on the cell type and passage number. Start any new
transfection by testing the recommended four concentrations of Sirius to
determine an optimum amount. Stability: 24 months.
Order Information
Name | Catalog | Size |
Sirius Transfection Reagent | P400025 | 0.25ml |
Sirius Transfection Reagent | P400050 | 0.5ml |
Sirius Transfection Reagent | P400075 | 0.75ml |
Sirius Transfection Reagent | P400150 | 1.5ml |
Sirius Transfection Reagent | P400500 | 5.0ml |
Sirius Transfection Reagent | P401000 | 10.0ml |
